超声法提取抱石莲中绿原酸_汪启炉

本资料包含pdf文件1个，下载需要20积分

超声法提取抱石莲中绿原酸汪启炉摘要：目的探讨抱石莲中绿原酸的最佳提取工艺。方法建立高效液相法测定绿原酸含量，采用超声波提取的方
法对药材进行提取，以抱石莲中绿原酸得量为参考指标，采用单因素实验、正交实验和方差分析对提取工艺进行考
察♂果绿原酸含量检测回归方程为 Y3.72×10
6
X－0.72×10
6 ，r0.999 7（7.8～390.0 μg • mL
－1）。最
佳提取工艺条件为：pH4.0，乙醇浓度70%，超声时间30 min，料液比1 : 12，超声提取2次，按此条件进行提取，
绿原酸得率为1.67%（n3）♂论利用单因素考察及正交试验的方法优化绿原酸的提取工艺，为抱石莲中绿
原酸的工业化提韧生产奠定了基矗
关键词：绿原酸；抱石莲；正交试验；提取工艺
中图分类号：R284文献标识码：A文章编号：1672-2981(2013)01-0019-05
doi:10.7539/j.issn.1672-2981.2013.01.006
Extracting chlorogenic acid from Lepidogrammitis drymoglossoides
(Baker) Ching by ultrasound method
WANG Qi-lu
1
， WEN Jia-gen
2
， CHEN Yu-xiang
3
， LI Gang
1
(1. Department of Medical Apparatus， No.94 Hospi-tal of the PLA， Nanchang 330002; 2. Institute of Clinical Pharmacology， Central South University， Changsha
410008; 3.National Hepatobiliary and Enteric Surgery Research Center of The Ministry of Health， Central
South University， Changsha 410008)
Abstract: Objective To investigate the optimal extraction process of chlorogenic acid (CGA) from Lepidogrammitis
drymoglossoides(Baker) Ching.MethodsHPLC was used to determine the quantity of CGA. Ultrasound extraction was
used. Single factor experiment， orthogonal experiments and analysis of variance were all taken to optimize the extraction
process， with CGA content as the index.ResultsThe regression curve for CGA analysis was Y3.72×10
6
X－0.72×10
6，
r0.999 7(7.8－390.0 μg • mL
－1
). The optimal conditions for extracting were as follows: pH4.0， 70% ethanol con-centration， 30 min ultrasound extraction， 1 : 12 solid-liquid ratio， twice. The yield of CGA was 1.67% (n3). Conclusion
Single factor experiment and orthogonal test can help optimize the extraction process of chlorogenic acid， which lays the
foundation for industrial extraction and production of CGA from Lepidogrammitis drymoglossoides.
Key words: chlorogenic acid; lepidogrammitis drymoglossoides; orthogonal test; extraction craft